Species Specificity of Amino Acid Acceptor Ribonucleic Acid and Aminoacyl Soluble Ribonucleic Acid Synthetases
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چکیده
منابع مشابه
Species Specificity of Amino Acid Acceptor Ribonucleic Acid and Aminoacyl Soluble Ribonucleic Acid Synthetases.
Several investigators have reported that the activation of amino acids for protein synthesis, i.e. the incorporation of amino acids into soluble ribonucleic acids, is dependent upon the source of both the aminoacyl S-RNA’ synthetases and the S-RNA itself. For example, Rendi and Ochoa (1) reported that aminoacyl S-RNA synthetases from Escherichia coli could not incorporate leucine into yeast or ...
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A procedure for preparing polyribosome aminoacyl-tRNA free from contamination by supernatant aminoacyl-tRNA and free amino acids is described. Important features of the procedure are the use of acidic buffers to help protect the amino acid-tRNA linkage and the inclusion ofsodium dodecyl sulphate, to inhibit ribonuclease activity. The specific radioactivity of polyribosome aminoacyl-tRNA is high...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1963
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)75325-3